Benzotriazole alleviates copper mediated lysosomal membrane damage and antioxidant defense system responses in earthworms (Eisenia fetida).

Benzotriazole (BTR) is a common corrosion inhibitor used to protect copper (Cu) and Cu alloys. To reveal the combined subacute toxicity of BTR and Cu at environmental levels on terrestrial animals, the activity of antioxidative enzymes and the glutathione levels in earthworms (Eisenia fetida) of the single or co-exposure treatments were determined. The activity of both antioxidant enzymes and non-enzymatic antioxidants was affected by BTR in earthworms. Moreover, the analyses of lysosomal neutral red retention time and total antioxidant capacity indicated a detoxification effect of BTR on Cu-induced impairments of the antioxidant defense capacity in earthworms. The apoptotic rate of coelomocytes in earthworms of the co-exposure treatment was lower than that in earthworms treated with Cu only, indicating that BTR alleviates Cu mediated lysosomal membrane damage and antioxidant defense system responses in earthworms.

Effects of benzotriazole on copper accumulation and toxicity in earthworm (Eisenia fetida).

Triazole contaminants in water and soil environments can form complexes with metal ions, and therefore affect the bioavailability and toxicity of some heavy metals. In present study, significant increase of copper (Cu) uptake by earthworm (Eisenia fetida) was observed when combined pollution of benzotriazole (BTR) presented in soil. For instance, Cu accumulation in earthworms increased 55% approximately when BTR presented at the BTR/Cu molar ratio of 1:2.5. While the single Cu exposure (at 32 mg kg-1 in soil) resulted in increased malondialdehyde (MDA) content in earthworms from 0.319 to 0.668 nmol mg protein-1, joint exposure to BTR at BTR/Cu molar ratio of 1:10 significantly decreased the MDA content to 0.405 nmol mg protein-1. This indicates a potential detoxification effect of BTR to Cu induced oxidative damage in earthworms. Varied Cu subcellular distribution can be observed in earthworms of the single and combined exposure treatments. With the combined exposure of BTR, the proportion of Cu associated with granular fraction, the toxically inert fraction in earthworms, increased from 25% to 39%. This phenomenon can be used to explain the protective effects of BTR against oxidative damage.

Effects of single and combined exposures to copper and benzotriazole on Eisenia fetida.

Benzotriazole (BTR), an emerging class of environmental pollutant, is widely used in industrial applications and household dishwashing agents. Despite the reported toxicity of BTR to aquatic organisms, little is known about its effects on terrestrial invertebrates. Copper (Cu) accumulates in agricultural soils receiving urban waste products, fertilizers, fungicides, and urban sewage. In this study, two different types of bioassays (acute toxicity test and behavioral toxicity test) were performed to evaluate the toxicity of Cu and BTR, both singly and together, on the earthworm (Eisenia fetida) in artificial soil. The results of avoidance behaviour tests showed that the EC50,48 h values for Cu and BTR were 1.47 and 0.46 mmol kg-1, respectively. The results of the acute toxicity tests showed that the LC50,7 d and LC50,14 d of Cu in earthworms were 9.19 and 5.28 mmol kg-1, respectively, and the LC50,7 d and LC50,14 d of BTR were 2.43 and 1.76 mmol kg-1, respectively. Toxicity analysis demonstrated that the binary BTR and Cu mixture had predominantly antagonistic effects on the avoidance behaviour and survival of earthworms. The Cu2+ activities and mortality of earthworms decreased significantly with increasing concentrations of BTR, while the solid-liquid distribution coefficient of Cu increased. These results indicated that the presence of BTR can reduce the toxicity as well as the bioavailability of Cu in soil with both BTR and Cu.

Effect of corrosion inhibitor benzotriazole on the uptake and translocation of Cd in rice (Oryza sativa L.) under different exposure conditions.

Emerging contaminants that can complex with heavy metals might affect the speciation of coexisting metals and result in different ecological risks. As a widely used metal corrosion inhibitor, 1H-benzotriazole (BTR) is frequently detected in the environments, sometimes at very high levels. In this study, rice (Oryza sativa L.) was used to assess the ecological risk of combined exposure to cadmium (Cd) and BTR in plants and discuss the potential effects of exposure sequence on the uptake and translocation of Cd under hydroponic culture. In the combined exposure treatments, Cd concentration in rice significantly decreased when the molar ratio of BTR to Cd exceeded 1, while the oxidative damage of root was alleviated. In the sequential exposure treatments, an exposure to BTR accelerated the release of preabsorbed Cd from seedlings to the environment and increased the transport of Cd from the roots to shoots at high BTR concentrations. This demonstrates that the combined pollution effect of Cd and BTR is present not only in the environment but also in plants. With the decrease in Cd concentration in the roots, the electrolytic leakages from the roots also decreased, indicating that root damage repair was induced by the subsequent BTR exposure. BTR was mainly accumulated in the seedling roots. Preabsorbed BTR significantly increased Cd concentration in the roots of rice seedlings but inhibited Cd translocation from the roots to shoots of the rice seedlings.

Hepatotoxicity of benzotriazole and its effect on the cadmium induced toxicity in zebrafish Danio rerio.

As an emerging contaminant, 1-H-benzotriazole (1H-BTR) has been detected in the engineered and natural aquatic environments, which usually coexists with heavy metals and causes combined pollution. In the present study, wild-type and transgenic zebrafish Danio rerio were used to explore the acute toxicity as well as the single and joint hepatotoxicity of cadmium (Cd) and 1H-BTR. Although the acute toxicity of 1H-BTR to zebrafish was low, increased expression of liver-specific fatty acid binding protein was observed in transgenic zebrafish when the embryos were exposed to 5.0 µM of 1H-BTR for 30 days. Besides, co-exposure to 1H-BTR not only reduced the acute toxic effects induced by Cd, but also alleviated the Cd-induced liver atrophy in transgenic fish. Correspondingly, effects of combined exposure to 1H-BTR on the Cd-induced expressions of several signal pathway-related genes and superoxide dismutase and glutathione-s-transferase proteins were studied. Based on the determination of Cd bioaccumulation in fish and the complexing stability constant (ß) of Cd-BTR complex in solution, the detoxification mechanism of co-existing 1H-BTR on Cd to the zebrafish was discussed.